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Flow cytometric analysis of apoptosis : DNA ploidy and apoptosis were analyzed using fluorescence activated cell sorting (FACS) flow cytometer (Becton Dickinson, Sunnyvale, CA) ultram equipped with a 15 mW air-cooled 488 nm argon-ion laser .
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A total of 20 000 events were recorded in list mode and analyzed using the Cell Quest Pro software (Becton Dickinson) at Mansoura ultram University Hospital .
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