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Histological investigations: Testis and ovaries of both control and experimental groups were incised immediately, fixed in 10% phosphate tramadol hydrochloride high dosage tramadol dose for joint pain tramadol med tramadol pills for pain buffered formalin (pH 7 . is tramadol a scheduled drug in texas opiate withdrawal gnc tramadol schedule ky tramadol 50 mg high Serial 5 µm thick sections were cut and stained with Haematoxylin and eosin (H&E), examined under bright field light microscopy, and photographed . 4), dehydrated in ascending grades of ethyl alcohol, cleared in xylene and mounted in molten pararplast ultram 58−62 °C .
Flow cytometric analysis of apoptosis : DNA ploidy and apoptosis were analyzed using fluorescence activated cell sorting (FACS) flow cytometer (Becton Dickinson, Sunnyvale, CA) ultram equipped with a 15 mW air-cooled 488 nm argon-ion laser .
FL1 (FITC) signals were detected through can you crush tramadol pills tramadol and alcohol and weed tramadol 50mg zydol tramadol zaldiar a 530/30 nm band-pass filter; FL2 (PI) signals were detected through a 585/42 nm band-pass filter .
A total of 20 000 events were recorded in list mode and analyzed using the Cell Quest Pro software (Becton Dickinson) at Mansoura ultram University Hospital .
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